  
                                  SEQUENCE LISTING
  
  <110> WARF - Wisconsin Alumni Research Foundation
       Watanabe, Tokiko
        Kawaoka, Yoshihiro
  
  <120> INFLUENZA M2 PROTEIN MUTANT VIRUSES AS LIVE INFLUENZA ATTENUATED
       VACCINES
    
  
  <130> 800.055WO1
  
  <150> US 60/944,680       
  <151> 2007-06-18  
  
  <160> 53
  
  <170> FastSEQ for Windows Version 4.0
  
  <210> 1
  <211> 19
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 1
  aagagggtca cttgaatcg                                              19
  
  <210> 2
  <211> 18
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 2
  actgttgctg cgagtatc                                               18
  
  
  
  <210> 3
  <211> 18
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 3
  gttgttgctc caactatc                                               18
  
  <210> 4
  <211> 18
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 4
  gttgttgctg cgaacatc                                               18
  
  
  <210> 5
  <211> 22
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 5
  gttgttatca ttgggatctt gc                                          22
  
  <210> 6
  <211> 18
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 6
  cccaatgata ctcgcagc                                               18
  
  <210> 7
  <211> 25
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 7
  atcttgcact tgatattggc aattc                                       25
  
  <210> 8
  <211> 62
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 8
  caccagtgaa ctggcgacag ttgagtagat cgccagaatg tcacttgaat cgttgcatct 60
  gc                                                                62
  
  <210> 9
  <211> 57
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 9
  cttttggtct ccctgggggc aatcagtttc tggatggatc gtcttttttt caaatgc    57
  
  <210> 10
  <211> 68
  <212> DNA
  <213> Artificial Sequence
  
  
  <220> 
  <223> A synthetic primer
  
  <400> 10
  gcttagtatc aattgtattc catttatgat tgatatccaa atgctgtcac ttgaatcgtt 60
  gcatctgc                                                          68
  
  <210> 11
  <211> 63
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 11
  attataggag tcgtaatgtg tatctcaggg attaccataa tagatcgtct ttttttcaaa 60
  tgc                                                               63
  
  <210> 12
  <211> 33
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligonucleotide
  
  <400> 12
  gggttattgg agacggtacc gtctcctccc ccc                              33
  
  <210> 13
  <211> 18
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligonucleotide
  
  <220>
  <221> misc_feature   
  <222> 7
  <223> n = A, T, G, or C
  
  <400> 13
  cgtctcntat tagtagaa                                               18
  
  <210> 14
  <211> 16
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligonucleotide
  
  <220>
  <221> misc_feature   
  <222> 11
  <223> n = A, T, G, or C
  
  <400> 14
  ttttgctccc ngagac                                                 16
  
  <210> 15
  <211> 11
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligonucleotide
  
  <400> 15
  tattagtaga a                                                      11
  
  <210> 16
  <211> 10
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligonucleotide
  
  <400> 16
  gggagcaaaa                                                        10
  
  
  <210> 17
  <211> 15
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligonucleotide
  
  <400> 17
  gggttattag tagaa                                                  15
  
  <210> 18
  <211> 13
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligonucleotide
  
  <400> 18
  ttttgctccc ccc                                                    13
  
  <210> 19
  <211> 80
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 19
  cacacacgtc tcgtattagt agaaacaagg tcgtttttaa actattcgac actaattgat 60
  ggccatccga attcttttgg                                             80
  
  <210> 20
  <211> 67
  <212> DNA
  <213> Artificial Sequence
  
  
  <220> 
  <223> A synthetic primer
  
  <400> 20
  cacacacgtc tccgggagcg aaagcaggtc aattatattc aatatggaaa gaataaaaga 60
  actaagg                                                           67
  
  <210> 21
  <211> 89
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 21
  cacacacgtc tcgtattagt agaaacaagg cattttttca tgaaggacaa gctaaattca 60
  ctatttttgc cgtctgagct cttcaatgg                                   89
  
  <210> 22
  <211> 67
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 22
  cacacacgtc tccgggagcg aaagcaggca aaccatttga atggatgtca atccgacttt 60
  acttttc                                                           67
  
  <210> 23
  <211> 103
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 23
  ccaacccgtc tcctattagt agaaacaagg tacttttttg gacagtatgg atagcaaata 60
  gtagcattgc cacaactatc tcaatgcatg tgtgaggaag gag                   103
  
  
  
  <210> 24
  <211> 67
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 24
  ccaacccgtc tccgggagcg aaagcaggta ctgattcaaa atggaagatt ttgtgcgaca 60
  atgcttc                                                           67
  
  <210> 25
  <211> 40
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 25
  cacacacgtc tcctattagt agaaacaagg gtgtttttcc                       40
  
  <210> 26
  <211> 45
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 26
  cacacacgtc tccgggagca aaagcagggg aaaataaaaa caacc                 45
  
  <210> 27
  <211> 47
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 27
  cacacacgtc tcctattagt agaaacaagg gtatttttct ttaattg               47
  
  <210> 28
  <211> 42
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 28
  cacacacgtc tccgggagca aaagcagggt agataatcac tc                    42
  
  <210> 29
  <211> 46
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 29
  cacacacgtc tcctattagt agaaacaagg agttttttga acaaac                46
  
  <210> 30
  <211> 48
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 30
  cacacacgtc tccgggagcg aaagcaggag tttaaatgaa tccaaacc              48
  
  <210> 31
  <211> 47
  <212> DNA
  <213> Artificial Sequence
  
  
  <220> 
  <223> A synthetic primer
  
  <400> 31
  cacacacgtc tcctattagt agaaacaagg tagtttttta ctccagc               47
  
  <210> 32
  <211> 41
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 32
  cacacacgtc tccgggagca aaagcaggta gatattgaaa g                     41
  
  <210> 33
  <211> 53
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 33
  cacacacgtc tcctattagt agaaacaagg gtgtttttta ttattaaata agc        53
  
  <210> 34
  <211> 46
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 34
  cacacacgtc tccgggagca aaagcagggt gacaaagaca taatgg                46
  
  
  
  
  <210> 35
  <211> 28
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 35
  gtgaatagaa ttggagtaaa aaactacc                                    28
  
  <210> 36
  <211> 27
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 36
  tcaaaaatga ccatcgtcaa catccac                                     27
  
  
  <210> 37
  <211> 28
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 37
  gtgagatggt cattttgtca acatagaa                                    28
  
  <210> 38
  <211> 27
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 38
  tcaatccaca gcactctgct gttcctg                                     27
  
  <210> 39
  <211> 26
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 39
  gtgacggcag gaacagcaga gtgctg                                      26
  
  <210> 40
  <211> 25
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 40
  tcattccctc atagactcag gtacc                                       25
  
  <210> 41
  <211> 25
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 41
  gtgagcaggg gtacctgagt ctatg                                       25
  
  <210> 42
  <211> 24
  <212> DNA
  <213> Artificial Sequence
  
  
  <220> 
  <223> A synthetic primer
  
  <400> 42
  tcaaggccct cttttcaaac cgta                                        24
  
  <210> 43
  <211> 29
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 43
  cttaaatacg gtttgaaaag agggcctgc                                   29
  
  <210> 44
  <211> 34
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 44
  tcactcaata aatgcatttg aagaaaagac gatc                             34
  
  <210> 45
  <211> 26
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 45
  ttgttgttgc cgcaaatatc attggg                                      26
  
  
  
  
  <210> 46
  <211> 28
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic primer
  
  <400> 46
  ttcactcaac ttgaatcgct gcatctgc                                    28
  
  <210> 47
  <211> 10
  <212> PRT
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligopeptide
  
  <220>
  <221> SITE           
  <222> 10
  <223> Xaa = R or G
  
  
  <400> 47
  Pro Gln Arg Glu Arg Arg Arg Lys Lys Xaa
   1               5                  10  
  
  
  <210> 48
  <211> 6
  <212> PRT
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligopeptide
  
  <220>
  <221> SITE           
  <222> 6
  <223> Xaa = R or G
  
  <400> 48
  Pro Gln Arg Glu Thr Xaa
   1               5      
  
  
  <210> 49
  <211> 16
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligopeptide
  
  <220>
  <221> misc_feature   
  <222> 6
  <223> n = A, T, G, or C
  
  <400> 49
  gtctcnggga gcaaaa                                                 16
  
  <210> 50
  <211> 15
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligopeptide
  
  <400> 50
  ttctactaat aaccc                                                  15
  
  <210> 51
  <211> 13
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligopeptide
  
  <400> 51
  ggggggagca aaa                                                    13
  
  
  <210> 52
  <211> 33
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligopeptide
  
  <400> 52
  ggggggagga gacggtaccg tctccaataa ccc                              33
  
  <210> 53
  <211> 18
  <212> DNA
  <213> Artificial Sequence
  
  <220> 
  <223> A synthetic oligopeptide
  
  <220>
  <221> misc_feature   
  <222> 12
  <223> n = A, T, G, or C
  
  <400> 53
  ttctactaat angagacg                                               18
